Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/srep21385
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Funding
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R21AI098701]
- Canadian Institutes
- Fundacion la Caixa (Spain)
- Fundacion Canada (Spain)
- Fundacion Ramon Areces (Spain)
- Fundacao para a Ciencia e a Tecnologia-Ministerio da Educacao e Ciencia (FCT-MEC, Portugal) [SPRH/BD/100517/2014]
- Marie Sklodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE, European Union) [644167]
- CNPq
- CAPES
- FUNDECT
- FAPDF
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Due to the growing concern about antibiotic-resistant microbial infections, increasing support has been given to new drug discovery programs. A promising alternative to counter bacterial infections includes the antimicrobial peptides (AMPs), which have emerged as model molecules for rational design strategies. Here we focused on the study of Pa-MAP 1.9, a rationally designed AMP derived from the polar fish Pleuronectes americanus. Pa-MAP 1.9 was active against Gram-negative planktonic bacteria and biofilms, without being cytotoxic to mammalian cells. By using AFM, leakage assays, CD spectroscopy and in silico tools, we found that Pa-MAP 1.9 may be acting both on intracellular targets and on the bacterial surface, also being more efficient at interacting with anionic LUVs mimicking Gram-negative bacterial surface, where this peptide adopts alpha-helical conformations, than cholesterol-enriched LUVs mimicking mammalian cells. Thus, as bacteria present varied physiological features that favor antibiotic-resistance, Pa-MAP 1.9 could be a promising candidate in the development of tools against infections caused by pathogenic bacteria.
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