4.5 Article

Time-Dependent Anabolic Response of hMSC-Derived Cartilage Grafts to Hydrostatic Pressure

Related references

Note: Only part of the references are listed.
Article Biophysics

An assessment of the response of human MSCs to hydrostatic pressure in environments supportive of differential chondrogenesis

Farhad Chariyev-Prinz et al.

Summary: Mechanical stimulation can modulate the chondrogenic differentiation of stem/progenitor cells, but its effect on functional articular cartilage engineering using human mesenchymal stem cells (hMSCs) may not be necessary if other culture conditions have been optimized. This study investigated the impact of cell seeding densities and fibrin concentrations on chondrogenesis of hMSCs and found that high cell densities were more supportive of sGAG deposition, while lower fibrin concentrations promoted more robust chondrogenesis. Mechanical stimulation had limited effect on cartilage-specific matrix production, except when hMSCs were first primed with TGF-beta 3. These findings suggest that mechanical stimulation may not be essential for engineering functional articular cartilage grafts, but can be used to understand tissue response to mechanical loading in vivo.

JOURNAL OF BIOMECHANICS (2023)

Article Biotechnology & Applied Microbiology

Hydrostatic Pressure Regulates the Volume, Aggregation and Chondrogenic Differentiation of Bone Marrow Derived Stromal Cells

Paola Aprile et al.

Summary: This study aimed to investigate the regulation of chondrogenesis of mesenchymal stem cells. It was found that adjusting the stiffness of the hydrogel matrix and timing of hydrostatic pressure exposure can modulate the chondrogenic process of MSCs. These findings provide new insights into cartilage tissue regeneration.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

Article Biology

A single day of TGF-β1 exposure activates chondrogenic and hypertrophic differentiation pathways in bone marrow-derived stromal cells

Kathryn Futrega et al.

Summary: The study challenges the long-standing belief that BMSC differentiation requires over 2 weeks of continuous TGF-beta 1 exposure, showing that differentiation can be triggered by just a single day of exposure through the use of a micro-pellet model.

COMMUNICATIONS BIOLOGY (2021)

Article Multidisciplinary Sciences

Metabolic reprogramming in chondrocytes to promote mitochondrial respiration reduces downstream features of osteoarthritis

Yoshifumi Ohashi et al.

Summary: Metabolic dysfunction in chondrocytes is associated with the pro-catabolic phenotype of osteoarthritic cartilage, and replacing glucose with galactose can reverse this pattern and block detrimental changes related to osteoarthritis. The findings suggest that targeting mitochondrial metabolism may offer a novel approach to osteoarthritis treatment.

SCIENTIFIC REPORTS (2021)

Review Medicine, Research & Experimental

The role of microRNAs in the osteogenic and chondrogenic differentiation of mesenchymal stem cells and bone pathologies

Maria Rosa Iaquinta et al.

Summary: Mesenchymal stem cells (MSCs) play a crucial role in tissue engineering and regenerative medicine for bone tissue and cartilage. The regulation of miRNAs is important for MSCs differentiation, and dysregulation of miRNAs may be associated with osteoporosis, bone fractures, and bone cancer. Bone-associated circulating miRNAs could be useful biomarkers for diagnosis, follow-up, and treatment of cancer and metastases.

THERANOSTICS (2021)

Article Biochemical Research Methods

Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip

Simone Perottoni et al.

Summary: The stem cell niche in the perivascular space of human tissue is crucial for determining stem cell fate, with mesenchymal stem cells experiencing specific metabolic conditions. Current models lack advanced simulation of disruptive conditions, while organ-on-a-chip technology provides tools for simulating stem cell niches. The proposed metabolic toolbox combines in vitro systems with high-resolution imaging to monitor cell metabolism in real-time, serving as a functional tool for simulating the perivascular niche.

LAB ON A CHIP (2021)

Article Biotechnology & Applied Microbiology

Stiffness Matters: Fine-Tuned Hydrogel Elasticity Alters Chondrogenic Redifferentiation

Barbara Bachmann et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Review Biochemistry & Molecular Biology

The Importance of Physioxia in Mesenchymal Stem Cell Chondrogenesis and the Mechanisms Controlling Its Response

Girish Pattappa et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

Article Cell & Tissue Engineering

CELLS UNDER PRESSURE - THE RELATIONSHIP BETWEEN HYDROSTATIC PRESSURE AND MESENCHYMAL STEM CELL CHONDROGENESIS

G. Pattappa et al.

EUROPEAN CELLS & MATERIALS (2019)

Article Cell Biology

TGF-β Family Signaling in Mesenchymal Differentiation

Ingo Grafe et al.

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2018)

Review Engineering, Biomedical

Biomaterials for articular cartilage tissue engineering: Learning from biology

A. R. Armiento et al.

ACTA BIOMATERIALIA (2018)

Article Cell & Tissue Engineering

Engineering large cartilage tissues using dynamic bioreactor culture at defined oxygen conditions

Andrew C. Daly et al.

JOURNAL OF TISSUE ENGINEERING (2018)

Review Multidisciplinary Sciences

Mechanobiology of limb musculoskeletal development

Varun Arvind et al.

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (2017)

Article Cell Biology

Transcriptional Control by the SMADs

Caroline S. Hill

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2016)

Article Rheumatology

Mitochondrial respiration and redox coupling in articular chondrocytes

Rachel S. Lane et al.

ARTHRITIS RESEARCH & THERAPY (2015)

Review Rheumatology

Fracture healing: mechanisms and interventions

Thomas A. Einhorn et al.

NATURE REVIEWS RHEUMATOLOGY (2015)

Review Rheumatology

Repair and tissue engineering techniques for articular cartilage

Eleftherios A. Makris et al.

NATURE REVIEWS RHEUMATOLOGY (2015)

Article Multidisciplinary Sciences

Separating NADH and NADPH fluorescence in live cells and tissues using FLIM

Thomas S. Blacker et al.

NATURE COMMUNICATIONS (2014)

Article Engineering, Biomedical

Cell-matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure

A. J. Steward et al.

ACTA BIOMATERIALIA (2012)

Article Orthopedics

Effects of in vivo mechanical loading on large bone defect regeneration

Joel D. Boerckel et al.

JOURNAL OF ORTHOPAEDIC RESEARCH (2012)

Article Engineering, Biomedical

The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells

E. G. Meyer et al.

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS (2011)

Review Pathology

Lack of oxygen in articular cartilage: consequences for chondrocyte biology

Jerome E. Lafont

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (2010)

Article Biochemistry & Molecular Biology

Transforming Growth Factor β Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors

Katharine H. Wrighton et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Article Cell & Tissue Engineering

The Effect of Hydrostatic Pressure on Three-Dimensional Chondroinduction of Human Adipose-Derived Stem Cells

Rei Ogawa et al.

TISSUE ENGINEERING PART A (2009)

Article Endocrinology & Metabolism

ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes

Kenneth W. Finnson et al.

JOURNAL OF BONE AND MINERAL RESEARCH (2008)

Article Cell Biology

TGF-β signaling is essential for joint morphogenesis

Anna Spagnoli et al.

JOURNAL OF CELL BIOLOGY (2007)

Article Orthopedics

In vivo cyclic axial compression affects bone healing in the mouse tibia

Michael J. Gardner et al.

JOURNAL OF ORTHOPAEDIC RESEARCH (2006)

Article Cell Biology

Growth factor impact on articular cartilage subpopulations

EM Darling et al.

CELL AND TISSUE RESEARCH (2005)