Haploid male fertility is restored byparallel spindle genes inArabidopsisthaliana

Doubled haploid technology can accelerate plant breeding and its two main steps are haploid induction and subsequent doubled haploid production from fertile haploid plants. Although haploid female fertility is present to some extent in plants, the lack of haploid male fertility is a bottleneck. Herein, we demonstrate that mutations in the parallel spindle genes are sufficient to restore haploid male fertility in Arabidopsiswith no impact on haploid female fertility.

Automated summary

Doubled haploid technology is an efficient method for accelerating plant breeding, with key steps involving haploid induction and doubled haploid production from fertile haploid plants. The lack of haploid male fertility has been a major limitation, but this study showcases that mutations in parallel spindle genes can overcome this limitation in Arabidopsis, restoring haploid male fertility without affecting haploid female fertility.