Journal
CHEMICAL COMMUNICATIONS
Volume 59, Issue 28, Pages 4185-4188Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d3cc00897e
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Here we introduce a novel approach using a europium(iii) complex to monitor the transmembrane transport of fluoride into liposomes. By utilizing the long emission lifetime of this probe, we are able to accurately measure the transport activity of fluorescent transporters. The high sensitivity, selectivity, and versatility of this assay enable us to investigate various types of fluoride transporters and unravel their mechanisms of action.
Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(iii) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of the assay allowed us to study different types of fluoride transporters and unravel their mechanisms of action.
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