Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume -, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1007/s00253-023-12723
Keywords
Nanomaterials; European corn borer; Bioassay; Bioprotectant; Cry toxicity; Environmental degradation
Categories
Ask authors/readers for more resources
Nanotechnology was used to encapsulate Bt insecticidal protein Cry1Ab, enhancing its stability under environmental conditions. Two emulsions stabilized by Cu2+-SQDs/S-CN nanocomposites and GO nanosheets were prepared, and a pH-sensitive polymer was incorporated to allow Cry1Ab release in alkaline pH. The results showed that the nanomaterials effectively protected Cry1Ab from degradation and increased its pesticidal activity.
Nanotechnology is a promising way to enhance the stability of Bacillus thuringiensis (Bt) insecticidal proteins under envi-ronmental conditions. In this work, two emulsions were prepared through the Pickering emulsion technique, stabilized by Cu2+-SQDs/S-CN nanocomposites and by GO nanosheets. In addition, a pH-sensitive polymer was incorporated into these emulsions, allowing the Bt protein, Cry1Ab, to be released in an alkaline pH environment, as it occurs in the lepidopteran pests' gut. The effectiveness of these two nanomaterials in protecting Cry1Ab from degradation, and therefore enhancing its pesticidal activity, was assessed by exposing samples of the purified unprotected protein and encapsulated protein to high-intensity UV light and 40 degrees C temperature treatments. The UV treatment results were evaluated using SDS-PAGE analysis and pointed out that Cry1Ab could be structurally protected by the emulsions. The bioassays with first instar larvae of the lepidopteran pest Ostrinia nubilalis confirm the nanomaterial protection to UV and temperature treatments, i.e., decreasing about half the degradation rate and increasing up to 12-fold the residual activity after UV treatment. Our results indicate that encapsulation could be an effective strategy to improve the effectiveness of Cry1Ab under environmental conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available