Carbon sequestration performance, enzyme and photosynthetic activity, and transcriptome analysis of algae-bacteria symbiotic system after antibiotic exposure

Wastewater treatment technology based on algae-bacteria successfully combines pollutant purification, CO2 reduction and clean energy production to provide new insights into climate solutions. In this study, the reciprocal mechanisms between algae and bacteria were explored through physiological and biochemical levels of algae cells and differentially expressed genes (DEGs) based on the performance of immobilized algae-bacteria symbiotic particles (ABSPs) for CO2 fixation. The results showed that ABSPs promoted the CO2 fixation capacity of microalgae. The enhanced growth capacity and photosynthetic activity of algal cells in ABSPs are key to promoting CO2 uptake, and the stimulation of photosynthetic system and the promotion of Calvin cycle were the main contributors to enhanced carbon sequestration. These findings will provide guidance for carbon reduction using immobilized ABSS as well as deciphering the algae-bacteria reciprocal mechanism.

Automated summary

Wastewater treatment technology based on algae-bacteria combines pollutant purification, CO2 reduction, and clean energy production, providing new insights into climate solutions. This study explores the reciprocal mechanisms between algae and bacteria, focusing on the CO2 fixation capacity of immobilized algae-bacteria symbiotic particles (ABSPs) through physiological and biochemical analyses. The results demonstrate that ABSPs enhance the CO2 fixation capacity of microalgae by promoting growth and photosynthetic activity, primarily through activation of the photosynthetic system and promotion of the Calvin cycle.