Journal
RSC ADVANCES
Volume 6, Issue 64, Pages 59865-59874Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ra09081h
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Funding
- Malaysian Ministry of Science, Technology and Innovation (MOSTI) [03-01-05-SF0568, PJKIMIA/6013387]
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Lactose hydrolysis and transgalactosylation catalyzed by beta-galactosidase are important for the production of galacto-oligosaccharides (GOS). These reactions were performed in an enzymatic membrane reactor (EMR) with beta-galactosidase immobilized on the inner lumen of the membrane. The effects of hydrodynamic parameters of transmembrane pressure (TMP) and nominal feed flow rate on the reaction were investigated. The rate of GOS formation increased as TMP was increased from 0.2 bar to 0.5 bar and then decreased as the TMP was further increased to 0.8 bar. In contrast to that of GOS, the formation rate of monosaccharides significantly decreased at high nominal feed flow rates. The separation factor between GOS and monosaccharides increased at high TMP, but the overall permeation flux decreased because of the high membrane resistant coefficient of membranes with immobilized beta-galactosidase. Moreover, the specific productivity of trisaccharides was higher, whereas that of tetrasaccharides was lower in the EMR than that in batch reactor systems.
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