Journal
CLINICAL ORAL INVESTIGATIONS
Volume 20, Issue 4, Pages 675-683Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00784-015-1556-7
Keywords
Periodontitis; Rheumatoid arthritis; Citrullination; Porphyromonas gingivalis; Citrullinated peptide antibodies
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Funding
- German Society of Periodontology (DGParo/GABA)
- US NIH [DE 022597]
- European Commission [FP7-PEOPLE-2011-ITN-290246 RAPID, FP7-HEALTH-F3-2012-306029 TRIGGER]
- Polish National Centre of Science project [OPUS 2012/05/B/NZ6/00581]
- Polish Ministry of Science and Higher Education [2975/7.PR/13/2014/2]
- Ministry of Science and Higher Education
- German Academic Exchange Service [314-D/08/48763]
- German Society of Periodontology
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Objectives The aim of the present study was to assess human and bacterial peptidylarginine deiminase (PAD) activity in the gingival crevicular fluid (GCF) in the context of serum levels of antibodies against citrullinated epitopes in rheumatoid arthritis and periodontitis. Materials and methods Human PAD and Porphyromonas gingivalis-derived enzyme (PPAD) activities were measured in the GCF of 52 rheumatoid arthritis (RA) patients (48 with periodontitis and 4 without) and 44 non-RA controls (28 with periodontitis and 16 without). Serum antibodies against citrullinated epitopes were measured by ELISA. Bacteria being associated with periodontitis were determined by nucleic-acid-based methods. Results Citrullination was present in 26 (50 %) RA patients and 23 (48 %) controls. PAD and PPAD activities were detected in 36 (69 %) and 30 (58 %) RA patients, respectively, and in 30 (68 %) and 21 (50 %) controls, respectively. PPAD activity was higher in RA and non-RA patients with periodontitis than in those without (p = 0.038; p = 0.004), and was detected in 35 of 59 P. gingivalis-positive samples, and in 16 of 37 P. gingivalis-negative samples in association with high antibody levels against that species. Conclusions PAD and PPAD activities within the periodontium are elevated in RA and non-RA patients with periodontitis. PPAD secreted by P. gingivalis residing in epithelial cells may exert its citrullinating activity in distant regions of the periodontium or even distant tissues.
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