Journal
RSC ADVANCES
Volume 6, Issue 115, Pages 114019-114023Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ra24122k
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Funding
- National Natural Science Foundation of China [21342012, 21305042]
- Scientific Research Fund of Hunan provincial Science and Technology Department [14JJ4030]
- Opening Fund of Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Hunan Normal University) Ministry of Education [KLCBTCMR2011-08]
- Introduce Talents Fund of Hunan Agricultural University [13YJ01]
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This work focuses on a facile method for antibody immobilization to fabricate a label-free electrochemical immunosensor using ferrocene-containing thiol aromatic aldehyde (FcSA) synthesized by us. The FcSA plays important roles as follows: provides a novel self-assembled monolayer and the aldehyde group was applied to immobilize antibodies directly, in which no additional activation step was required. Ferrocene can improve the electrical conductivity of the sensing interface as compared to the ferrocene-free thiol aromatic aldehyde. Human IgG as a model analyte was detected in the range from 0.01 to 40 ng mL(-1) with a detection limit of 0.005 ng mL(-1) based on a signal-to-noise ratio (S/N) of 3. The immunosensor showed good specificity, acceptable reproducibility as well as satisfactory stability.
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