Journal
ONCOTARGET
Volume 8, Issue 7, Pages 11566-11578Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.14141
Keywords
brigatinib; ATC; ceritinib; crizotinib; neuroblastom
Categories
Funding
- Swedish Cancer Society [15/391, 15/775]
- Swedish Childhood Cancer Foundation [2015-0096, 201580, 2014-150, TJ2016-0088, NC2014-0045]
- Swedish Research Council [2015-04466, BH 521-2012-2831]
- Goran Gustafsson Foundation (RHP)
- SSF Programme Grant [RB13-0204]
- Postdoctoral Research Fellow Position
- Vinnova [2015-04466] Funding Source: Vinnova
- Swedish Research Council [2015-04466] Funding Source: Swedish Research Council
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Activating mutations in full length anaplastic lymphoma kinase (ALK) have been reported in neuroblastoma and in anaplastic thyroid cancer. ALK-L1198F and ALK-G1201E mutations were originally identified in anaplastic thyroid cancer (ATC) and characterized as constitutively activating mutations. In this study, we employed in vitro cell culture assays together with biochemical and in vivo Drosophila analyses to characterize their sensitivity to either activation by the FAM150A (AUG-beta) and FAM150B (AUG-alpha) ALK ligands or inhibition by ALK inhibitors. Here we report that neither ALK-L1198F nor ALK-G1201E mutations result in ligand independent gain-of-function (GOF) activity in either in vitro biochemical analysis or the various model systems employed. ALK-L1198F is activated by the FAM150 (AUG) ligands and its ligand-dependant activity is similar to the wild type full length ALK receptor. ALKG1201E is only very weakly activated by the FAM150 (AUG) ligands, most likely due to impaired protein stability. We conclude that neither ALK-L1198F nor ALK-G1201E displays ligand independent kinase activity, with ALK-L1198F belonging to the class of ligand dependent ALK mutations which are not constitutively active but that responds to ligand activation, while the ALK-G1201E mutation generates an unstable receptor with very low levels of kinase activity.
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