Journal
ONCOTARGET
Volume 7, Issue 32, Pages 51934-51942Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.10623
Keywords
chloramphenicol; multiple myeloma; adenosine triphosphate; apoptosis
Categories
Funding
- Science and Technology R&D Fund of Shenzhen [JCYJ 20140414123738256]
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Chloramphenicol is an old antibiotic that also inhibits mammalian mitochondrial protein synthesis. Our studies demonstrated that chloramphenicol is highly cytotoxic to myeloma cells, acting in a dose-and time-dependent manner. Chloramphenicol sharply suppressed ATP levels in myeloma cells at concentrations >= 25 mu g/mL. Colorimetric and clonogenic assays indicate that chloramphenicol inhibits growth of myeloma cell lines at concentrations >= 50 mu g/mL, and inhibits primary myeloma cell growth at concentrations >= 25 mu g/mL. Flow cytometry and Western blotting showed that chloramphenicol induces myeloma cell apoptosis at concentrations >= 50 mu g/mL. Chloramphenicol increased levels of cytochrome c, cleaved caspase-9 and cleaved caspase-3, suggesting that myeloma cell apoptosis occurs through the mitochondria-mediated apoptosis pathway. It thus appears chloramphenicol is not only an old antibiotic, it is also a potential cytotoxic agent effective against myeloma cells. This suggests chloramphenicol may be an effective new drug for the treatment of myeloma.
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