Journal
ONCOTARGET
Volume 7, Issue 24, Pages 36577-36589Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.9288
Keywords
gastric cancer; miRNA; miR-31; E2F2; biomarker
Categories
Funding
- National Natural Science Foundation of China [81320108025, 81472662]
- foundation of Jilin Province Science and Technology Department [20140414048GH]
- foundation of Jilin province education department [2016453]
- foundation of the Norman Bethune Program of Jilin University [2012219]
- foundation of Jilin university youth corps committee
- Graduate Innovation Fund of Jilin University [2015056]
- Jilin University - Xinjiang Medical University joint research project
Ask authors/readers for more resources
The miRNA microarray analysis showed that miR-31 was reduced in gastric cancer. This study further assessed miR-31 expression and role of miR-31 in gastric cancer tissues and cell lines. The data showed that miR-31 expression was down-regulated in 40 cases of gastric cancer tissues compared to the adjacent normal tissues, and low expression of miR-31 was associated with poor tumor differentiation, lymph node metastasis, advanced T stage and worse overall survival of gastric cancer patients. Ectopic expression of miR-31 reduced tumor cell viability, enhanced apoptosis, arrested tumor cells at G1 transition, and reduced tumor cell migration and invasion in SGC-7901 and MGC-803 gastric cell lines in vitro. Enforced expression of miR-31 also inhibited growth of engrafted tumors in vivo. Luciferase reporter assays and western blot revealed that E2F2 is the direct target of miR-31. E2F2 expression was upregulated in gastric cancer tissues, and inversely associated with miR-31 levels, while knockdown of E2F2 expression mimicked miR-31 anti-tumor activity in gastric cancer cells, but the ectopic expression of E2F2 rescued the miR-31-mediated inhibition in gastric cell lines. Taken together, these results demonstrated that miR-31 acts as a crucial tumor suppressive activity by inhibiting E2F2s expression. Thus, miR-31 might be a candidate therapeutic target for gastric cancer patients.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available