[64Cu]XYIMSR-06: A dual-motif CAIX ligand for PET imaging of clear cell renal cell carcinoma

Carbonic anhydrase IX (CAIX) is a cell surface enzyme that is over-expressed in approximately 95% of cases of clear cell renal cell carcinoma (ccRCC), the most common renal cancer. We synthesized and performed in vitro and in vivo evaluation of a dual-motif ligand, [Cu-64]XYIMSR-06, for imaging CAIX expression on ccRCC tumors using positron emission tomography (PET). [Cu-64] XYIMSR-06 was generated in yields of 51.0 +/- 4.5% (n=5) and specific activities of 4.1 - 8.9 GBq/mu mol (110-240 Ci/mmol). Tumor was visualized on PET images by 1 h post-injection with high tumor-to-background levels (>100 tumor-to-blood and - muscle) achieved within 24 h. Biodistribution studies demonstrated a maximum tumor uptake of 19.3% injected dose per gram of radioactivity at 4 h. Tumor-to-blood, - muscle and - kidney ratios were 129.6 +/- 18.8, 84.3 +/- 21.0 and 2.1 +/- 0.3, respectively, at 8 h post-injection. At 24 h a tumor-to-kidney ratio of 7.1 +/- 2.5 was achieved. These results indicate pharmacokinetics superior to those of previously reported imaging agents binding to CAIX. [Cu-64] XYIMSR-06 is a new low-molecular-weight PET ligand targeting CAIX, which can image localized and metastatic ccRCC.