Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 23, Issue 12, Pages 1111-1116Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.3321
Keywords
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Funding
- NIH [GM58650, R01GM064649]
- Program of the Presidium of the Russian Academy of Sciences 'Basic Research for the Development of Biomedical Technologies' [FIMT-2014-011]
- Ministry of Education and Science of the Russian Federation [14.621.21.0001, RFMEFI62114X0001]
- Russian Science Foundation [14-24-00031]
- M.V. Lomonosov Moscow State University Program of Development
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DNA accessibility to regulatory proteins is substantially influenced by nucleosome structure and dynamics. The facilitates chromatin transcription (FACT) complex increases the accessibility of nucleosomal DNA, but the mechanism and extent of its nucleosome reorganization activity are unknown. Here we determined the effects of FACT from the yeast Saccharomyces cerevisiae on single nucleosomes by using single-particle Forster resonance energy transfer (spFRET) microscopy. FACT binding results in dramatic ATP-independent, symmetrical and reversible DNA uncoiling that affects at least 70% of the DNA within a nucleosome, occurs without apparent loss of histones and proceeds via an 'all-or-none' mechanism. A mutated version of FACT is defective in uncoiling, and a histone mutation that suppresses phenotypes caused by this FACT mutation in vivo restores the uncoiling activity in vitro. Thus, FACT-dependent nucleosome unfolding modulates the accessibility of nucleosomal DNA, and this activity is an important function of FACT in vivo.
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