Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 23, Issue 9, Pages 803-810Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.3270
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Funding
- NIH [R01GM089846, 5P50GM103297]
- Austrian Science Fund (FWF) [P26550, P28725]
- China Scholarship Council
- Austrian Science Fund (FWF) [P28725] Funding Source: Austrian Science Fund (FWF)
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The B-DNA double helix can dynamically accommodate G-C and A-T base pairs in either Watson-Crick or Hoogsteen configurations. Here, we show that G-C+ (in which + indicates protonation) and A-U Hoogsteen base pairs are strongly disfavored in A-RNA. As a result, N-1-methyladenosine and N-1-methylguanosine, which occur in DNA as a form of alkylation damage and in RNA as post-transcriptional modifications, have dramatically different consequences. Whereas they create G-C+ and A-T Hoogsteen base pairs in duplex DNA, thereby maintaining the structural integrity of the double helix, they block base-pairing and induce local duplex melting in RNA. These observations provide a mechanism for disrupting RNA structure through post-transcriptional modifications. The different propensities to form Hoogsteen base pairs in B-DNA and A-RNA may help cells meet the opposing requirements of maintaining genome stability, on the one hand, and of dynamically modulating the structure of the epitranscriptome, on the other.
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