Journal
CONSERVATION GENETICS RESOURCES
Volume 8, Issue 3, Pages 231-234Publisher
SPRINGER
DOI: 10.1007/s12686-016-0541-z
Keywords
Species-specific primers; Real-time PCR; Lotic system; Species detection
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The freshwater crayfish, Cambaroides japonicus, is endangered in Hokkaido, Japan and inhabits burrows. Here, we applied environmental DNA (eDNA) method for evaluating the species distributions with comparing hand-capture method in the headwater streams. We detected the eDNA of C. japonicus from all sites, where we collected C. japonicus, and confirmed that eDNA can be applied to detect burrowing aquatic freshwater crayfish without disturbing their habitats.
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