Journal
CONSERVATION GENETICS RESOURCES
Volume 8, Issue 2, Pages 115-118Publisher
SPRINGER
DOI: 10.1007/s12686-016-0523-1
Keywords
eDNA; Environmental DNA; Detection; Species-specific; Redfin perch; Perca fluviatilis
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Funding
- Invasive Animals Cooperative Research Centre [1.W.2]
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Invasive alien species are one of the leading causes of extinctions worldwide. Preventing their establishment, eradicating or containing their spread relies on low-density detection. Environmental DNA (eDNA) has been shown to have superior detection sensitivity compared to traditional methods, permitting detection at lower densities. Here, we develop a species-specific assay to detect Perca fluviatilis (redfin perch), an invasive freshwater fish present in Australia. We show that the assay is highly sensitive and highly specific to detect the species in Australian freshwater environments and demonstrate the utility of the assay for detecting the species from environmental water samples. This assay will prove useful for low-density detection of P. fluviatilis to assist in the management of this invader, with conservation benefits for the native species it threatens.
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