Journal
BIOPRESERVATION AND BIOBANKING
Volume 14, Issue 4, Pages 289-297Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/bio.2015.0054
Keywords
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Funding
- Mais Centro-Programa Operacional Regional do Centro under the Quadro de Referencia Estrategico Nacional (QREN)
- Mais Centro-Programa Operacional Regional do Centro through the Fundo Europeu de Desenvolvimento Regional (FEDER)
- Fundacao para a Ciencia e Tecnologia (FCT) [PTDC/NEUNMC/0205/2012, UID/NEU/04539/2013]
- COMPETE Programa Operacional Factores de Competitividade, QREN
- European Union (FEDER-Fundo Europeu de Desenvolvimen to Regional)
- National Mass Spectrometry Network (RNEM) [REDE/1506/REM/2005]
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Biobank saliva sample quality depends on specific criteria applied to collection, processing, and storage. In spite of the growing interest in saliva as a diagnostic fluid, few biobanks currently store large collections of such samples. The development of a standard operating procedure (SOP) for saliva collection and quality control is fundamental for the establishment of a new saliva biobank, which stores samples to be made available to the saliva research community. Different collection methods were tested regarding total volume of protein obtained, protein content, and protein profiles, and the results were used to choose the best method for protein studies. Furthermore, the impact of the circadian variability and inter- and intraindividual differences, as well as the saliva sample stability at room temperature, were also evaluated. Considering our results, a sublingual cotton roll method for saliva collection proved to produce saliva with the best characteristics and should be applied in the morning, whenever possible. In addition, there is more variability in salivary proteins between individuals than in the same individual for a 5-month period. According to the electrophoretic protein profile, protein stability is guaranteed for 24 hours at room temperature and the protein degradation profile and protein identification were characterized. All this information was used to establish an SOP for saliva collection, processing, and storage in a biobank. We conclude that it is possible to collect saliva using an easy and inexpensive protocol, resulting in saliva samples for protein analysis with sufficient quality for biobanking purposes.
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