Journal
BIOMEDICAL OPTICS EXPRESS
Volume 7, Issue 11, Pages 4450-4471Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.7.004450
Keywords
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Funding
- New Growth Engine Industry Project of the Ministry of Knowledge and Economy [10047579]
- National Research Foundation of Korea (NRF) grant - Korea government (MSIP) [2015R1A2A2A03005382]
- GIST Research Institute (GRI)
- National Research Foundation of Korea [2015R1A2A2A03005382] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Understanding light intensity and temperature increase is of considerable importance in designing or performing in vivo optogenetic experiments. Our study describes the optimal light power at target depth in the rodent brain that would maximize activation of light-gated ion channels while minimizing temperature increase. Monte Carlo (MC) simulations of light delivery were used to provide a guideline for suitable light power at a target depth. In addition, MC simulations with the Pennes bio-heat model using data obtained from measurements with a temperature-measuring cannula having 12.3 mV/degrees C of thermoelectric sensitivity enabled us to predict tissue heating of 0.116 degrees C/mW on average at target depth of 563 mu m and specifically, a maximum mean plateau temperature increase of 0.25 degrees C/mW at 100 mu m depth for 473 nm light. Our study will help to improve the design and performance of optogenetic experiments while avoiding potential over-and under-illumination. (C) 2016 Optical Society of America
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