Journal
BIOMEDICAL OPTICS EXPRESS
Volume 7, Issue 9, Pages 3478-3490Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.7.003478
Keywords
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Funding
- National Natural Science Foundation of China [81327802, 81171151, 81501945]
- National High Technology Research and Development Program 863 Project [2015AA020501]
- National Funds for Distinguished Young Scientists [81525009]
- Shanghai Municipal Science and Technology Commission of Outstanding Academic Leaders Project [12XD1401400]
- Training Project of Excellent Talents in Shanghai Municipal Health System [WSJ2012-20]
- Shanghai Science and Technology Committee Sailing Program [14YF1400800]
- Shanghai Municipal Health Bureau Youth Fund [2012WSJ188]
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We introduce a more flexible optogenetics-based mapping system attached on a stereo microscope, which offers automatic light stimulation to individual regions of interest in the cortex that expresses light-activated channelrhodopsin-2 in vivo. Combining simultaneous recording of electromyography from specific forelimb muscles, we demonstrate that this system offers much better efficiency and precision in mapping distinct domains for controlling limb muscles in the mouse motor cortex. Furthermore, the compact and modular design of the system also yields a simple and flexible implementation to different commercial stereo microscopes, and thus could be widely used among laboratories. (C) 2016 Optical Society of America
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