Journal
ACS COMBINATORIAL SCIENCE
Volume 18, Issue 6, Pages 287-291Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscombsci.6b00039
Keywords
peptoids; high-throughput; antimicrobial; combinatorial library
Funding
- Faculty Research and Creative Activity Committee (FRCAC)
- MTSU
- National Institutes of Health [R03 AI112861]
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Rapid emergence of antimicrobial resistant organisms necessitates equally rapid methods for the development of new antimicrobial compounds. Of recent interest have been mimics of antimicrobial peptides known as, antimicrobial peptoids, which exhibit similar potency to the former but with improved proteolytic stability. Presented herein is a high-throughput method to screen libraries of antimicrobial peptoids immobilized on beads embedded into solid media. Termed the peptoid library agar diffusion (PLAT) assay, this assay allows for individual chemical - manipulation of two identical peptoid strands. One strand can be released to diffuse out from a solid support bead and interact with the microorganism during screening. The other strand can be cleaved after screening from beads showing strong antimicrobial activity and analyzed by mass spectrometry to deconvolute the structure of the peptoid. This method was applied to a small library of peptoids to identify an antimicrobial peptoid with modest efficacy against the ESKAPE pathogens.
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