Journal
ACS CATALYSIS
Volume 7, Issue 1, Pages 710-724Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscatal.6b02979
Keywords
biocatalysis; enzymatic cascades; in vitro biotransformations; in vivo biotransformations; biocatalytic retrosynthesis; whole-cell biocatalysis; enzymes; cofactors
Categories
Funding
- BBSRC [sLoLa BB/L502005/1]
- BBSRC (CASE/Pfizer)
- Royal Society
- BBSRC [BB/M028836/1, BB/M017702/1, BB/K00199X/1, BB/M029034/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [1394023, BB/M029034/1, BB/M017702/1, E20061/2, BB/K00199X/1, 1782874, BB/M028836/1] Funding Source: researchfish
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The combination of sequential biocatalytic reactions, via non-natural synthetic cascades, is a rapidly developing field and leads to the generation of complex valuable chemicals from simple precursors. As the toolbox of available biocatalysts continues to expand, so do the options for biocatalytic retrosynthesis of a target molecule, leading to alternative routes employing enzymatic transformations. The implementation of such cascade reactions requires careful consideration, particularly with respect to whether the pathway is constructed in vitro or in vivo. In this Perspective, we describe the relative merits of in vitro, in vivo, and hybrid approaches to building biocatalytic cascades and showcase recent developments in the area. We also highlight the factors that influence the design and implementation of purely enzymatic or chemoenzymatic, one-pot, multistep pathways.
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