Journal
NATURE COMMUNICATIONS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms12524
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Funding
- NeCEN, the Netherlands Centre for Electron Nanoscopy, Leiden, NL
- NWO, the Netherlands Organisation for Scientific Research
- European Regional Development Fund of the European Commission
- Spanish Ministry of Economy and Competitiveness [AIC-A-2011-0638, BIO2013-44647-R]
- Instruct, European Strategy Forum on Research Infrastructures (ESFRI)
- Instruct Image Processing Center I2PC
- NanoNextNL of the Government of the Netherlands
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In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the asymmetric virion structure of bacteriophage MS2, which includes 178 copies of the coat protein, a single copy of the A-protein and the RNA genome. This reveals that in situ, the viral RNA genome can adopt a defined conformation. The RNA forms a branched network of stem-loops that almost all allocate near the capsid inner surface, while predominantly binding to coat protein dimers that are located in one-half of the capsid. This suggests that genomic RNA is highly involved in genome packaging and virion assembly.
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