Journal
NATURE COMMUNICATIONS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms10534
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Funding
- Instruct part of the European Strategy Forum on Research Infrastructures (ESFRI) [1300]
- Instruct Image Processing Center I2PC
- NZ Health Research Council
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Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 angstrom resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.
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