Journal
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
Volume 8, Issue 12, Pages -Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/cshperspect.a023853
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Funding
- National Science Foundation (NSF) [MCB-0943393]
- Office of Naval Research [N00014-11-1-0363]
- Defense Advanced Research Projects Agency Young Faculty Award (DARPA YFA) Program [N66001-11-1-4137]
- Army Research Office [W911NF-11-1-0445]
- NSF Materials Network Grant [DMR-1108350]
- DARPA Living Foundries Program [N66001-12-C-4211]
- David and Lucile Packard Foundation [2011-37152]
- Advanced Research Projects Agency-Energy (ARPA-E) [DE-AR0000435]
- Chicago Biomedical Consortium
- Searle Funds at the Chicago Community Trust
- NSF Graduate Research Fellowship [DGE-1144469]
- Northwestern Biotechnology Training Program by the National Institutes of Health (NIH) [T32GM008449]
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Cell-free protein synthesis (CFPS) technologies have enabled inexpensive and rapid recombinant protein expression. Numerous highly active CFPS platforms are now available and have recently been used for synthetic biology applications. In this review, we focus on the ability of CFPS to expand our understanding of biological systems and its applications in the synthetic biology field. First, we outline a variety of CFPS platforms that provide alternative and complementary methods for expressing proteins from different organisms, compared with in vivo approaches. Next, we review the types of proteins, protein complexes, and protein modifications that have been achieved using CFPS systems. Finally, we introduce recent work on genetic networks in cell-free systems and the use of cell-free systems for rapid prototyping of in vivo networks. Given the flexibility of cell-free systems, CFPS holds promise to be a powerful tool for synthetic biology as well as a protein production technology in years to come.
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