Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 20, Issue -, Pages 486-495Publisher
ELSEVIER
DOI: 10.1016/j.jff.2015.11.030
Keywords
Lactobacillus; Dipeptidyl peptidase IV; DPP-IV inhibition; alpha-glucosidase inhibition; Probiotic; Diabetes
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Funding
- National Natural Science Foundation of China [31071507]
- National High Technology Research and Development Program (863 Program) [2008AA10Z310]
- Ministry of Science and Technology of the People's Republic of China [2011BAD09B03]
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Lactobacillus strains were screened for inhibition of dipeptidyl peptidase IV (DPP-IV) and alpha-glucosidase. Cell-free excretory supernatants (CFS) and cell-free extracts (CFE) of 21 strains were prepared, and most of them showed DPP-IV inhibition in a concentration-dependent manner, which started at the exponential phase and peaked at the stationary phase of the Lactobacillus. The CFS bioactivity was heat-resistant, stable to acid alkali and glucosidase treatment. Trypsin treatment specifically elevated CFS's DPP-IV inhibition. Lactobacillus plantarum strains CFS displayed different protein patterns and trypsin sensitive bands on SDS-PAGE, demonstrating specific peptide-cleavage contributes to higher inhibition. Seven strains with highest DPP-IV inhibition also displayed alpha-glucosidase inhibitory activity, of which, three strains of L. plantarum and one of Lactobacillus brevis showed tolerance to simulated gastrointestinal tract conditions and high HT-29 cell adhesion. This initial report of lactobacilli secreting strong DPP-IV and alpha-glucosidase inhibition components will be beneficial for selection and application of anti-diabetic probiotics. (C) 2015 Elsevier Ltd. All rights reserved.
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