4.7 Article

Modulation of the TNFα-induced gene expression profile of intestinal epithelial cells by soy fermented with lactic acid bacteria

Journal

JOURNAL OF FUNCTIONAL FOODS
Volume 23, Issue -, Pages 400-411

Publisher

ELSEVIER
DOI: 10.1016/j.jff.2016.02.047

Keywords

Soy milk; Lactic acid bacteria; Probiotic; Intestinal epithelial cell; TNF alpha; NF-kappa B

Funding

  1. Natural Sciences and Engineering Research Council of Canada [CRDPJ 35643-07]
  2. Canadian Space Agency

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The effect of soy ferments on gene expression induced by the proinflammatory cytokine tumour necrosis factor alpha (TNF alpha) at the intestinal epithelial cell (IEC) level was evaluated. Microarray-based transcriptional analysis revealed that soy fermented with Lactobacillus helveticus R0052 and Streptococcus thermophilus R0083 (SF-Lh) down-regulated 33 of 40 proinflammatory genes up-regulated by TNFa in HT-29 IEC, attenuating expression of genes encoding several proinflammatory cytokines and cell adhesion molecules. TNF alpha-mediated activation of gene expression associated with the NF-kappa B pathway was also repressed, as was Interleukin-8 (IL-8) production. In contrast, soy fermented with Bifidobacterium longum R0175 and S. thermophilus R0083 (SF-Bl) up-regulated expression of three proinflammatory genes induced by TNF alpha. Increased intracellular levels of hydrogen peroxide (H2O2) were detected in HT-29 IEC following incubation with SF-Lh. These results indicate that SF-Lh has immunomodulatory activity through reduction of proinflammatory gene expression at the IEC level. (C) 2016 Elsevier Ltd. All rights reserved.

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