Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 23, Issue -, Pages 24-39Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2016.02.021
Keywords
Common bean; Bioactive peptides; Colorectal cancer; Synergistic interaction; Oxaliplatin
Categories
Funding
- Consejo Nacional de Ciencia y Tecnologia CONACyT-Mexico [259497]
- CONACyT-Ciencia Basica grant [220924]
- University of Illinois - Universidad Autonoma de Queretaro research grant Hatch program [ILLU-698-384]
Ask authors/readers for more resources
The inhibitory effect of pure peptides from common bean on human colorectal cancer cells, their ability to enhance oxaliplatin effects, and mechanisms of action were evaluated. Peptides GLTSK, LSGNK, GEGSGA, MTEEY, and MPACGSS were tested for cytotoxic effects on HCT116 and CCD-33Co human normal colon cells; no peptide was toxic to normal cells. GLTSK (IC50 = 134.6 mu M) and GEGSGA (IC50 = 156.7 mu M) exerted anti-proliferative effects on HCT116 cells; LSGNK, MTEEY, and MPACGSS were less potent. GLTSK (gamma = 0.64) and GEGSGA (gamma = 0.78) interacted synergistically with oxaliplatin inhibiting HCT116 cells. GLTSK caused loss of mitochondrial potential (Delta psi m) (15.8%) and increased intracellular ROS (12.1-fold) suggesting mitochondrial membrane disruption. GEGSGA caused arrest in G1 phase (63.6% of cell population) and promoted cleavage of PARP (183.7%) suggesting DNA damage. GEGSGA and oxaliplatin caused activation and nuclear translocation of p53. Thus, peptides from common beans selectively induced apoptosis through loss of Delta psi m and DNA damage on human colorectal cancer cells. (C) 2016 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available