Oral administration of a casein matrix containing β-casofensin protects the intestinal barrier in two preclinical models of gut diseases

beta-Casofensin is a milk-derived bioactive peptide that interacts with intestinal goblet cells. We aimed to determine whether beta-casofensin could prevent intestinal dysfunctions induced by neonatal matemal separation (NMS) and whether it retains its protective effects when administered in a casein matrix. We also evaluated whether a casein matrix enriched in beta-casofensin protects against intestinal enteritis induced by indomethacin. beta-Casofensin abolished NMS-induced jejunal hyperpermeability and prevented the depletion of goblet and Paneth cells induced by NMS. In addition, beta-casofensin maintained its effectiveness against NMS-induced intestinal barrier alterations when administered in a casein matrix. A casein matrix containing beta-casofensin also reduced intestinal damages induced by indomethacin. A functional food containing beta-casofensin may prevent both the deleterious effects of neonatal stress on the intestinal barrier and indomethacin-induced enteritis. These results suggest a promising application of beta-casofensin as a gut barrier protector in the context of bioactive foods and clinical nutrition. (C) 2016 Elsevier Ltd. All rights reserved.