4.7 Article

Effects of L-paracasei subp. paracasei X12 on cell cycle of colon cancer HT-29 cells and regulation of mTOR signalling pathway

Journal

JOURNAL OF FUNCTIONAL FOODS
Volume 21, Issue -, Pages 431-439

Publisher

ELSEVIER
DOI: 10.1016/j.jff.2015.12.024

Keywords

Probiotics; Colon cancer; Cell cycle; mTOR signalling pathway

Funding

  1. project Harbin Outstanding Academic Leaders [RC2014XK020002]
  2. Harbin Institute of Technology Project [AUGA9833213611]

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L. paracasei subp. paracasei X12(1) is obtained from traditional cheese in northwestern China and exhibited effective anticancer activities in previous screening experiments. Here, its effects on colon cancer cell line HT-29 cell cycle and the roles of mammalian target of rapamycin (mTOR) signalling were investigated. Firstly, the effects of Lactobacillus X12 at different multiplicity of infection (MOI) on HT-29 cell cycle were detected by flow cytometry. In order to determine the specific regulators involved, cyclin D-1, cyclin E-1, the positive regulators, and p21, p16, and p27, the negative regulatory factors, were further explored by RT-PCR technique. Finally, mTOR signalling was focused to demonstrate its relationship with cell cycle arrest. Overall, the present findings suggested that Lactobacillus X12 can block HT-29 cell cycle at G(1) phase through inhibiting cyclin E-1, meanwhile enhancing p27, which were mediated by mTOR/4EBP(1)(2) signalling pathway. These results will provide a theoretical basis for further development of Lactobacillus. (C) 2015 Elsevier Ltd. All rights reserved.

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