Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 24, Issue -, Pages 403-419Publisher
ELSEVIER
DOI: 10.1016/j.jff.2016.04.020
Keywords
Mushroom; Agaricus bisporus; Aqueous enzymatic extracts; Liquid chromatography-tandem; mass spectrometry; Antiviral activity; HCV
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Funding
- Junta de Andalucia
- Spanish Ministerio de Economia y Competitividad (MINECO)
- FEDER program [FQM-1602, IPT-2011-1418-0]
- Ministerio de Ciencia e Innovacion (MICINN) through a Ramon y Cajal Contract [RYC-2009-03921]
- MECD [FPU14/03068]
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Aqueous enzymatic extracts of edible mushroom Agaricus bisporus (AbAEE) have been studied as a key source of biological active compounds, among which those with antiviral activity against HCV are of special interest. Searching for a better knowledge of AbAEE, a new approach based on sequential fractionation with solvents of different polarity is here proposed to improve the tentative identification of AbAEE components by liquid chromatography tandem mass spectrometry (LC-QTOF MS/MS) in high resolution mode. Fifty five out of one hundred twenty-three total metabolites tentatively identified by combination of MS and MS/MS information were identified for the first time. The newly identified compounds include amino acids, sugars, carboxylic, fatty and cinnamic acids, mono- and disaccharides, phospholipids, and purines as the most outstanding, thus demonstrating that fractionation based on sequential liquid-liquid extraction followed by LC-QTOF MS/MS is a suitable option to obtain a wide, representative snapshot of AbAEE composition. (C) 2016 Elsevier Ltd. All rights reserved.
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