Journal
CHEMICAL SCIENCE
Volume 7, Issue 3, Pages 2157-2161Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5sc02631h
Keywords
-
Categories
Funding
- National Key Scientific Program of China [2011CB911000]
- NSFC [NSFC 21221003, NSFC 21327009]
- China National Instrumentation Program [2011YQ03012412]
- National Institutes of Health [GM079359, GM111386, CA133086]
- NATIONAL CANCER INSTITUTE [R01CA133086] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM111386, R01GM079359] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Conjugation of DNA to defined locations on a protein surface will be a powerful tool for positioning functional groups and molecules in biological and biomedical studies. However, tagging protein with DNA is challenging in physiological environments, and requires a bioorthogonal approach. Here, we report a chemical solution to selectively conjugate DNA aptamers with a protein by protein-aptamer template (PAT)-directed reactions. Since protein-aptamer interactions are bioorthogonal, we exploit the PAT as a unique platform for specific DNA-protein cross-linking. We develop a series of modified oligonucleotides for PAT-directed reactions and find an F-carboxyl group as a suitable functionality for selective and site-specific conjugation. The functionality is incorporated into aptamers in our F-carboxyl phosphoramidite with an easy synthesis. We also demonstrate the necessity of a linker between the reactive functionality and the aptamer sequences.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available