Journal
CHEMICAL SCIENCE
Volume 7, Issue 8, Pages 4945-4950Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6sc00802j
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Funding
- National Natural Science Foundation of China [21335005, 21472120]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R43]
- Fundamental Research Funds for the Central Universities [GK201501003]
- 111 Project [B14041]
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Telomerase is a crucial biomarker for cancers. Its reliable and sensitive detection, particularly in a single cell, has great significance for the early diagnosis of cancers, studies on tumor progression and anticancer therapy, which remain a challenge, due to nonspecific amplification. Herein, we developed a novel stem-loop primer-mediated exponential amplification (SPEA) strategy, which can specifically and efficiently amplify the telomerase-elongated telomere repeat unit with near zero nonspecific signal. The SPEA-based assay can accurately detect telomerase activity in the crude lysate of a single cell and is suited for detecting the cellular heterogeneity arising from cell-to-cell variations.
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