Journal
CHEMICAL SCIENCE
Volume 7, Issue 2, Pages 1051-1056Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5sc03471j
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Categories
Funding
- 973 Program [2013CB933800]
- National Natural Science Foundation of China [21535004, 21227005, 21390411, 21275092, 21575081, 21405098]
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Hydrogen selenide (H2Se), a highly reactive Se species, is an important selenium metabolism intermediate involved in many physiological and pathological processes. This compound is of scientific interest with regard to the real-time monitoring of H2Se in living cells and in vivo to understand the anti-cancer mechanism of selenium. However, monitoring H2Se in living cells is still challenging due to the lack of straight forward, highly selective and rapid methods. Here, we developed a novel small-molecule fluorescent probe, NIR-H2Se, for imaging endogenous H2Se. NIR-H2Se exhibited high selectivity toward H2Se over selenocysteine (Sec), H2S and small molecule thiols and was successfully used to image the H2Se content in HepG(2) cells during Na2SeO3-induced apoptosis. Increased H2Se content and reduced ROS levels were observed under hypoxic conditions compared to normoxic conditions, which indicated that the cell apoptosis induced by Na2SeO3 under a hypoxic environment is via a non-oxidative stress mechanism. Thus, this probe should serve as a powerful tool for exploring the physiological function of H2Se and Se anticancer mechanisms in a variety of physiological and pathological contexts.
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