Journal
YONSEI MEDICAL JOURNAL
Volume 57, Issue 2, Pages 283-290Publisher
YONSEI UNIV COLL MEDICINE
DOI: 10.3349/ymj.2016.57.2.283
Keywords
Anti-bacterial agent; gentamicin; macrophage; phagocytosis
Categories
Funding
- Japan Society for the Promotion of Science [22300261, 22590388]
- Grants-in-Aid for Scientific Research [22300261, 22590388] Funding Source: KAKEN
Ask authors/readers for more resources
Macrophages (M phi) play a pivotal role in the protection system by recognizing and eliminating invading pathogenic bacteria. Phagocytosis and the killing of invading bacteria are major effector functions of M phi. Although the phagocytic and bactericidal activities of M phi have been analyzed via several methods using a light microscope, a fluorescence microscope, or a fluorescence-activated cell sorter, expensive materials and equipment are usually required, and the methods are rather complicated. Moreover, it is impossible to determine both the phagocytic and bactericidal activities of M phi simultaneously using these methods. In this review, we describe a simple, reproducible, inexpensive, yet old-fashioned method (antibiotic protection assay) for determining the phagocytic and bactericidal activities of M phi.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available