Journal
VIROLOGY
Volume 497, Issue -, Pages 157-162Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.07.015
Keywords
Arbovirus; Emerging disease; Flavivirus; Zika virus; Molecular clones; Recombinant virus; GFP reporter
Categories
Funding
- grant ZIKAVAx from the INSERM-transfert, France
- grant DIRED from the Conseil General de La Reunion, France
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Zika virus (ZIKV) infection is a major public health problem with severe human congenital and neurological anomalies. The screening of anti-ZIKV compounds and neutralizing antibodies needs reliable and rapid virus-based assays. Here, we described a convenient method leading to the rapid production of molecular clones of ZIKV. To generate a molecular clone of ZIKV strain MR766(NIID), the viral genome was directly assembled into Vero cells after introduction of four overlapping synthetic fragments that cover the full-length genomic RNA sequence. Such strategy has allowed the production of a recombinant ZIKV expressing the GFP reporter gene that is stable over two culturing rounds on Vero cells. Our data demonstrate that the ZIKV reporter virus is a very reliable GFP-based tool for analyzing viral growth and measuring the neutralizing antibody as well as rapid screening of antiviral effect of different classes of inhibitors. (C) 2016 Elsevier Inc. All rights reserved.
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