Journal
VIROLOGY
Volume 491, Issue -, Pages 20-26Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.01.005
Keywords
Arterivirus; Wobbly possum disease; WPD; Australian brushtail possum; Trichosurus vulpecula; Koch's postulates; Aetiology
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Funding
- Massey University Research Fund [RM15992]
- IVABS Postgraduate Research Fund [RM16192]
- McGeorge Research Fund [RM15763]
- Colin Holmes Dairy Scholarship
- George Mason Sustainable Land Use Scholarship
- Massey University Doctoral Scholarship
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The objective of this study was to investigate a role of a recently discovered marsupial nidovirus in the development of a neurological disease, termed wobbly possum disease (WPD), in the Australian brushtail possum (Trichosurus vulpecula). Four possums received 1 mL of a standard inoculum that had been prepared from tissues of WPD-affected possums, 4 possums received 1.8 mL (1 x 10(6) TCID50) of a cell lysate from inoculated cultures, and 4 possums received 1 mL (x 10(7) TCID50) of a purified WPD isolate. All but one possum that received infectious inocula developed neurological disease and histopathological lesions characteristic for WPD. High levels of viral RNA were detected in livers from all possums that received infectious inocula, but not from control possums. Altogether, our data provide strong experimental evidence for the causative involvement of WPD virus in development of a neurological disease in infected animals. (C) 2016 Elsevier Inc. All rights reserved.
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