Journal
VETERINARY JOURNAL
Volume 209, Issue -, Pages 174-179Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2015.10.032
Keywords
Silver nanoparticles; Antibacterial activity; Bacterial resistance; Synergy
Categories
Funding
- Ministry of Education, Youth and Sports of the Czech Republic [LO1305]
- Operational Programme Education for Competitiveness - European Social Fund of the Ministry of Education, Youth and Sports of the Czech Republic [CZ.1.07/2.3.00/20.0056]
- European Social Fund [CZ.1.07/2.4.00/31.0130]
- Czech Science Foundation [15-22248S]
- Internal Grants of Palacky University Olomouc [PrF_2015_022]
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Antibiotic resistant bacteria are a serious health risk in both human and veterinary medicine. Several studies have shown that silver nanoparticles (AgNPs) exert a high level of antibacterial activity against antibiotic resistant strains in humans. The aim of this study was to evaluate the antibacterial effects of a combined therapy of AgNPs and antibiotics against veterinary bacteria that show resistance to antibiotics. A microdilution checkerboard method was used to determine the minimal inhibitory concentrations of both types of antimicrobials, alone and in combination. The fractional inhibitory concentration index was calculated and used to classify observed collective antibacterial activity as synergistic, additive (only the sum of separate effects of drugs), indifferent (no effect) or antagonistic. From the 40 performed tests, seven were synergistic, 17 additive and 16 indifferent. None of the tested combinations showed an antagonistic effect. The majority of synergistic effects were observed for combinations of AgNPs given together with gentamicin, but the highest enhancement of antibacterial activity was found with combined therapy together with penicillin G against Actinobacillus pleuropneumoniae. A. pleuropneumoniae and Pasteurella multocida originally resistant to amoxycillin, gentamicin and colistin were sensitive to these antibiotics when combined with AgNPs. The study shows that AgNPs have potential as adjuvants for the treatment of animal bacterial diseases. (C) 2016 Elsevier Ltd. All rights reserved.
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