Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 169, Issue -, Pages 85-95Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2015.11.004
Keywords
Transcriptome sequencing; Chinese giant salamander; Aeromonas hydrophila; Bacterial infection; Immune modulation
Categories
Funding
- National Natural Science Foundation of China [31302221, 31272666]
- Jiangsu Province [BK2011418, BK20151297]
- Qinglan project of Jiangsu province of China
- BBSRC [BB/K009125/1]
- Biotechnology and Biological Sciences Research Council [BB/K009125/1] Funding Source: researchfish
- BBSRC [BB/K009125/1] Funding Source: UKRI
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The endangered Chinese giant salamander (Andrias davidianus) is the largest extant amphibian species. Disease outbreaks represent one of the major factors threatening A. davidianus populations in the wild and the viability of artificial breeding programmes. Development of future immune therapies to eliminate infectious disease in A. davidianus is dependent on a thorough understanding of the immune mechanisms elicited by pathogen encounters. To this end we have undertaken, for the first time in amphibians, differential transcriptome analysis of the giant salamander response to Aeromonas hydrophila, one of the most devastating pathogens affecting amphibian populations. Out of 87,204 non-redundant consensus unigenes 19,216 were annotated, 6834 of which were upregulated and 906 down-regulated following bacterial infection. 2058 unigenes were involved with immune system processes, including 287 differentially expressed unigenes indicative of the impact of bacterial infection on several innate and adaptive immune pathways in the giant salamander. Other pathways not directly associated with immune-related activity were differentially expressed, including developmental, structural, molecular and growth processes. Overall, this work provides valuable insights into the underlying immune mechanisms elicited during bacterial infection in amphibians that may aid in the future development of disease control measures in protecting the Chinese giant salamander. With the unique position of amphibians in the transition of tetrapods from aquatic to terrestrial habitats, our study will also be invaluable towards the further understanding of the evolution of tetrapod immunity. (C) 2015 Elsevier B.V. All rights reserved.
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