Journal
VECTOR-BORNE AND ZOONOTIC DISEASES
Volume 16, Issue 8, Pages 550-557Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/vbz.2014.1721
Keywords
Fluorescence microsphere immunoassay; IgM antibody response; Recombinant RVFV proteins; Rift Valley fever virus
Funding
- Science and Technology Directorate of the U.S. Department of Homeland Security
- Foreign Animal Disease Centers (Institute for Infectious Animal Diseases [IIAD] [HSHQDC-10-A-BOA33]
- Center of Excellence for Emerging and Zoonotic Animal Diseases [CEEZAD] [2010-ST061-AG0001]
- Kansas State NBAF Transition Funds
- USDA Agricultural Research [5430-32000-005-00D]
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A multiplex fluorescence microsphere immunoassay (FMIA) was used to detect bovine and ovine IgM and IgG antibodies to several Rift Valley fever virus (RVFV) proteins, including the major surface glycoprotein, Gn; the nonstructural proteins, NSs and NSm; and the nucleoprotein, N. Target antigens were assembled into a multiplex and tested in serum samples from infected wild-type RVFV or MP12, a modified live virus vaccine. As expected, the N protein was immunodominant and the best target for early detection of infection. Antibody activity against the other targets was also detected. The experimental results demonstrate the capabilities of FMIA for the detection of antibodies to RVFV structural and nonstructural proteins, which can be applied to future development and validation of diagnostic tests that can be used to differentiate vaccinated from infected animals.
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