Journal
VACCINE
Volume 34, Issue 9, Pages 1225-1231Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2015.11.001
Keywords
Vibrio alginolyticus; Vibrio harveyi; Vibrio parahaemolyticus; Cross immunogenic proteins; Immunoproteomic; DLD (dihydrolipoamide dehydrogenase)
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Funding
- National Natural Science Foundation of China [31402344]
- Guangdong Natural Science Foundation [S2013040014562]
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Vibrio spp. represent a serious threat to the culture of Epinephelus coioides (Orange-spotted Grouper) in Southeast Asia. In this study we used two-dimensional electrophoresis (2-DE) and Western blotting to identify common immunogenic proteins of Vibrio alginolyticus, Vibrio harveyi and Vibrio parahaemolyticus. Membranes were probed with orange-spotted grouper anti-V. alginolyticus sera and accordingly 60, 58 and 48 immunogenic protein spots were detected. By matching analysis for the three Western blotting membranes, 6 cross immunogenic spots for the three Vibrio species were identified. They were Outer membrane protein W (OmpW), dihydrolipoamide dehydrogenase (DLD), succinate dehydrogenase flavoprotein subunit(SDHA), elongation factor Ts(Ts), peptide ABC transporter periplasmic peptide-binding protein and phosphoenolpyruvate carboxykinase(PEPCK). One of the proteins, DLD, was used to evaluate the cross protective function for E. coioides with a bacterial immunization and challenge method. The relative percent survival rate of E. coioides against V. alginolyticus, V. harveyi and V. parahaemolyticus was 90%, 86% and 80%, respectively. This work may provide potential cross protective vaccine candidate antigens for three Vibrio species, and DLD may be considered as an effective cross-protective immunogen against three Vibrio species. (C) 2016 Published by Elsevier Ltd.
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