Journal
VACCINE
Volume 34, Issue 30, Pages 3508-3514Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2016.02.072
Keywords
Infectious bursal disease virus; Yeast; sVP
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Funding
- National Natural Science Foundation of China [31430087]
- Modern Agro-Industry Technology Research System [nycytx-42-G3-01]
- Application Technology Research and Development Fund of Harbin [2014AB3AN058]
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Infectious bursal disease virus (IBDV) causes immunosuppression in young chickens, leading to increased susceptibility to other diseases and a reduction in the immune response to other vaccines. Thus, IBDV results in great economic losses to the poultry industry. The most effective method of prevention is vaccination. However, medium-virulence vaccines can cause bursal pathological damage and immunosuppression. Here, we describe a safer, self-assembled, subvirus-like particle (sVP) vaccine without a complex purification process. The IBD-VP2 gene was cloned into Pichia pastoris, and the expressed protein self-assembled into T=1 sVPs (similar to 23 nm). Immunization experiments showed that the sVP vaccine elicited high IBDV-neutralizing antibodies in each group, and all birds survived challenge with very virulent IBDV (vvIBDV). Additionally, IBDV RNA was not detected, and sterile immunity was achieved. In conclusion, the IBD-sVP is a suitable candidate for a recombinant subunit vaccine against IBDV. (C) 2016 Published by Elsevier Ltd.
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