Journal
VACCINE
Volume 34, Issue 10, Pages 1312-1318Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2016.01.022
Keywords
Lactic acid bacteria; Protein vaccination; Fibronectin binding protein A (FnBPA); Human papillomavirus (HPV); HPV-16-E7 antigen
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Funding
- CAPES COFECUB project [720/11]
- CNPQScience without Border (Brazil)
- algerian government
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A recombinant strain of Lactococcus lactis displaying a cell-surface anchored fibronectin binding protein A (FnBPA) from Staphylococcus aureus (LL-FnBPA) had been shown to be more efficient in delivering plasmid than its wild-type counterpart both in vitro and in vivo, and have the ability to orientate the immune response toward a Th2 profile in a context of a DNA vaccination. The aim of this work was to test whether this LL-FnBPA strain could shape the immune response after mucosal administration in mice. For this, we used a mouse model of human papilloma virus (HPV)-induced cancer and a L. lactis strain displaying at its cell surface both HPV-16-E7 antigen (LL-E7) and FnBPA (LL-E7 + FnBPA). Our results revealed a more efficient systemic Th1 immune response with recombinant LL-E7 + FnBPA. Furthermore, mice vaccinated with LL-E7 + FnBPA were better protected when challenged with HPV-16-induced tumors. Altogether, the results suggest that FnBPA displays adjuvant properties when used in the context of mucosal delivery using L. lactis as a live vector. (C) 2016 Published by Elsevier Ltd.
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