Bleomycin enhances the efficacy of sonodynamic therapy using aluminum phthalocyanine disulfonate

Sonodynamic therapy (SDT), or ultrasound combined with sonosensitization, is a promising approach because it is noninvasive and penetrates deeper than light does in photodynamic therapy. We examined whether bleomycin (BLM) could improve the efficacy of SOT. We performed an in vitro study using Colon-26 cells, which are derived from mouse colon cancer. SDT with BLM was significantly more cytotoxic than SDT alone both in vitro and in vivo. We also observed an ultrasound intensity-dependent cytotoxic effect of SDT with BLM. These findings suggest that SOT with BLM might provide a novel noninvasive treatment for deep-seated tumors. (C) 2015 Elsevier B.V. All rights reserved.