Journal
ULTRASONICS
Volume 64, Issue -, Pages 43-53Publisher
ELSEVIER
DOI: 10.1016/j.ultras.2015.07.011
Keywords
Low intensity-pulsed ultrasound; SMMC-7721 cells; Apoptosis; Mitochondrial pathway; Calcium ion
Funding
- Research and Development Committee Program of the Zhongshan Hospital [Institute-005]
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The present study was conducted to determine whether low intensity-pulsed ultrasound (LIPUS) could induce apoptosis of human hepatocellular carcinoma cells, SMMC-7721, and to define the mechanism of ultrasound-induced apoptosis, in vitro. MTT assay was used to measure cell proliferation. Apoptosis was investigated by multiple methods such as flow cytometry, DNA fragmentation, Ca2+ mobilizations, pro- and anti-apoptotic protein expression, and light as well as ultramicroscopic morphology. The results provide evidence that LIPUS induced a dose-dependent effect on cell viability and apoptosis of SMMC-7721 cells. Specifically, exposure of cells to >0.5 Wicm2 intensity significantly increased cell apoptosis, caused shifts in cell cycle phase, and induced structural changes. Ultrasound significantly increased intracellular Ca2+ concentrations and modulated expression of caspase-3, Bcl-2 and Bax. The findings suggest that this novel technology can be used to induce SMMC-7721 apoptosis via the Ca2+/mitochondrial pathway and could potentially be of clinical use for the treatment of hepatocellular carcinoma (SMMC-7721 cell line) and other cancers. (C) 2015 Elsevier B.V. All rights reserved.
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