Journal
CLINICAL CHEMISTRY
Volume 62, Issue 1, Pages 48-69Publisher
AMER ASSOC CLINICAL CHEMISTRY
DOI: 10.1373/clinchem.2015.250563
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Funding
- Waters Inc.
- Thermo Inc.
- NIDDK [U01DK085689]
- NCI [U24CA115102]
- NIGMS [P50GM076547, R01GM087221]
- NCI CPTAC [U24CA160034, U24CA160019, U24CA160036, U24CA160035, U24CA159988]
- NATIONAL CANCER INSTITUTE [U24CA160019, U24CA160035, P30CA091842, U24CA159988, U24CA160036, U24CA115102, U24CA160034] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [U01DK085689] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM087221, P50GM076547] Funding Source: NIH RePORTER
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BACKGROUND: For many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotope labeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays. CONTENT: The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling, as well as approaches to the interpretation of the data generated by mass spectrometric protein assays. Additionally, the importance of carefully characterized reference materials in particular, peptide standards for the improved concordance of amino acid analysis methods across the industry is highlighted. The alignment of practices around the use of peptides and the transparency of sample preparation protocols should allow for the harmonization of peptide and protein quantification in research and clinical care. (C) 2015 American Association for Clinical Chemistry
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