Journal
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
Volume 15, Issue 7, Pages 1393-1400Publisher
PHARMACOTHERAPY GROUP
DOI: 10.4314/tjpr.v15i7.7
Keywords
Vaccine; Mycobacterium tuberculosis; Epitope prediction; Histocompatibility complexes
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Funding
- University of Malaya [PG238-2014B: PPP, RG064-12BIO, RG523/13HTM]
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Purpose: To investigate the expression of Myt272-3 recombinant protein and also to predict a possible protein vaccine candidate against Mycobacterium tuberculosis. Methods: Myt272-3 protein was expressed in pET30a+-Myt272-3 clone. The purity of the protein was determined using Dynabeads (R) His-Tag Isolation & Pulldown. Protein sequence was analysed in silico using bioinformatics software for the prediction of allergenicity, antigenicity, MHC-I and MHC-II binding, and B-cell epitope binding. Results: The candidate protein was a non-allergen with 15.19 % positive predictive value. It was also predicted to be antigenic, with binding affinity to MHC-I and MHC-II, as well as B-cell epitope binding. Conclusion: The predicted results obtained in this study provide a guide for practical design of a new tuberculosis vaccine.
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