Journal
TRENDS IN BIOTECHNOLOGY
Volume 34, Issue 4, Pages 329-345Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2015.12.008
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- Scientific and Technical Research Council of Turkey (TUBITAK-BIDEB)
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Bacillus subtilis is a highly promising production system for various biomolecules. This review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the approaches to design the initial production medium and/or feed stream. Additionally, the feeding strategies developed with or without feedback control for fed-batch B. subtilis fermentations were compiled with a special emphasis on recombinant protein (r-protein) production. For biomolecule production by wild-type B. subtilis, due to the different intracellular production patterns, no consensus exists on the FBO strategy that gives the maximum productivity, whereas for r-protein production appropriate feeding strategies vary depending on the promoter used. Thus, we conclude that the B. subtilis community is still seeking an approved strong promoter and generalized FBO strategies.
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