Journal
TOXICOLOGY
Volume 366, Issue -, Pages 1-9Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2016.08.001
Keywords
2-Chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine; Atrazine; Hypothalamus; Reproduction; Reptile
Categories
Funding
- NSF Integrative Organismal Systems Grant [0923300]
- NSF Division of Biological Infrastructure Grant [0959369]
- North Dakota EPSCoR through NSF grant [IIA-1355466]
- Academic Programs and Student Awards Committee, Department of Biology, University of North Dakota
- Esther Wadsworth Hall Wheeler Award, Department of Biology, University of North Dakota
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1558034, 0923300] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0959369] Funding Source: National Science Foundation
Ask authors/readers for more resources
Atrazine is an herbicide used to control broadleaf grasses and a suspected endocrine disrupting chemical. Snapping turtles lay eggs between late May and early June, which could lead to atrazine exposure via field runoff. Our goal was to determine whether a single exposure to 2 ppb or 40 ppb atrazine during embryogenesis could induce short- and long-term changes in gene expression within the hypothalamus of snapping turtles. We treated eggs with atrazine following sex determination and measured gene expression within the hypothalamus. We selected genes a priori for their role in the hypothalamus pituitary-gonad or the hypothalamus-pituitary-adrenal axes of the endocrine system. We did not identify any changes in gene expression 24-h after treatment. However, at hatching AR, Kiss1R, and POMC expression was upregulated in both sexes, while expression of CYP19A1 and PDYN was increased in females. Six months after hatching, CYP19A1 and PRLH expression was increased in animals treated with 2 ppb atrazine. Our study shows persistent changes in hypothalamic gene expression due to low-dose embryonic exposure to the herbicide atrazine with significant effects in both the HPG and HPA axes. Effects reported here appear to be conserved among vertebrates. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available