Journal
THORAX
Volume 71, Issue 12, Pages 1145-1153Publisher
BMJ PUBLISHING GROUP
DOI: 10.1136/thoraxjnl-2015-207402
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Funding
- Medical Research Council (UK)
- Wellcome Trust
- Fundacao Ciencia Tecnologia, Portugal
- Imperial College Healthcare Charity, UK
- Mason Medical Research Foundation UK
- NIHR Cambridge Biomedical Research Centre
- Imperial College Biomedical Research Centre UK
- MRC [MR/M009041/1, G1000183, G0802271, MR/M024873/1] Funding Source: UKRI
- Medical Research Council [G0802271, G1000183B, G1000183] Funding Source: researchfish
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Background It is unknown whether lesions in human TB are hypoxic or whether this influences disease pathology. Human TB is characterised by extensive lung destruction driven by host matrix metalloproteinases (MMPs), particularly collagenases such as matrix metalloproteinase-1 (MMP-1). Methods We investigated tissue hypoxia in five patients with PET imaging using the tracer [F-18]-fluoromisonidazole ([F-18]FMISO) and by immunohistochemistry. We studied the regulation of MMP secretion in primary human cell culture model systems in normoxia, hypoxia, chemical hypoxia and by small interfering RNA (siRNA) inhibition. Results [F-18]FMISO accumulated in regions of TB consolidation and around pulmonary cavities, demonstrating for the first time severe tissue hypoxia in man. Patlak analysis of dynamic PET data showed heterogeneous levels of hypoxia within and between patients. In Mycobacterium tuberculosis (M. tb)-infected human macrophages, hypoxia (1% pO(2)) upregulated MMP-1 gene expression 170-fold, driving secretion and caseinolytic activity. Dimethyloxalyl glycine (DMOG), a small molecule inhibitor which stabilises the transcription factor hypoxia-inducible factor (HIF)-1 alpha, similarly upregulated MMP-1. Hypoxia did not affect mycobacterial replication. Hypoxia increased MMP-1 expression in primary respiratory epithelial cells via intercellular networks regulated by TB. HIF-1 alpha and NF-kappa B regulated increased MMP-1 activity in hypoxia. Furthermore, M. tb infection drove HIF-1 alpha accumulation even in normoxia. In human TB lung biopsies, epithelioid macrophages and multinucleate giant cells express HIF-1 alpha. HIF-1 alpha blockade, including by targeted siRNA, inhibited TB-driven MMP-1 gene expression and secretion. Conclusions Human TB lesions are severely hypoxic and M. tb drives HIF-1 alpha accumulation, synergistically increasing collagenase activity which will lead to lung destruction and cavitation.
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